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The threshold optical density that discriminated staining from background was decided and kept constant for all those quantifications Fluorescent detection of major Abs was performed using Alexa Fluor 555 goat anti rabbit IgG (Life technologies, Grand Island, NY) at a 1:500 dilution for anti-PPD Ab, and Alexa flour 488 or 555 Donkey anti-mouse IgG (Life technologies) at 1:500 for anti-human CD4, CD8, and CD68, and anti-mouse F4/80 Abs